中国稻米

中国稻米 ›› 2016, Vol. 22 ›› Issue (4): 35-38.DOI: 10.3969/j.issn.1006-8082.2016.04.010

• 专论与研究 • 上一篇    下一篇

水稻抗二化螟特性鉴定技术的评价研究

  1. 1中国水稻研究所，杭州 310006；2 浙江大学，杭州 310000
  • 出版日期:2016-07-20 发布日期:2016-07-20
  • 通讯作者: 罗炬
  • 基金资助:

    农业部转基因重大专项（2014ZX08001-001，2016ZX08001-001）

Evaluation of Appraisement Method for Rice Resistance to Striped Stem Borer

  • Online:2016-07-20 Published:2016-07-20

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被引次数

1

摘要：

采用离体叶片、离体茎秆、离体剥开茎秆及活体成株4种室内抗虫性鉴定方法，对相同水稻材料进行抗二化螟特性鉴定，同时对这些水稻室内抗二化螟鉴定技术进行了综合评价。结果表明，这些方法的抗性鉴定结果高度吻合，离体叶片鉴定方法是目前最快速、有效的抗二化螟鉴定方法。并通过离体叶片和活体成株鉴定方法对转Vip3A基因水稻材料进行鉴定，再次验证了离体叶片鉴定方法的有效性。此外，初步发现对于同为鳞翅目水稻害虫稻纵卷叶螟，用离体叶片鉴定方法也可进行有效鉴定。

关键词: 水稻, 二化螟, 抗性鉴定, 技术评价

Abstract:

The identification result was consistent with the resistance evaluation to striped stem borer through four different bioassay methods, including detached-leaf bioassay, detached-stem bioassay, stripping detached-stem bioassay and whole plant bioassay. The detached-leaf bioassay is considered as the most rapid and effective method for evaluating resistance ability to striped stem borer. By both detached-leaf and whole plant bioassays, the author identified transgenic rice with Vip3A gene, and further validated the identification effectiveness of the detached-leaf bioassay method. Simultaneously, our preliminary results revealed that detached-leaf bioassay could also perform an accurate identification to rice leaf roller as lepidoptera pests of rice.

Key words: rice, striped stem borer, resistance evaluation, technology appraisement

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